Proteomics & Metabolomics

The core provides a range of equipment - from  protein imaging systems to mass-spectrometers - to support the study of proteomics and metabolomics.

Staff in charge: Ms. Joyce Wong and Ms. Sheree Lam

Liquid Chromatography & Mass Spectrometry

ÄKTAavant is a preparative liquid chromatography system intended for protein sample purification. On the front side of the instrument, ÄKTAavant has a built-in fraction collector with a cooling functionality that prevents overheating of purified samples.

UltrafleXtreme can be used for pure protein characterization and mass determination of peptide molecules. It can also determine mass of proteins or other biomolecules with high accuracy and resolution.

The timsTOF Pro achieves near 100% duty cycle for shotgun proteomics, by using the Parallel Accumulation Serial Fragmentation (PASEF) acquisition method in its dual TIMS (Trapped Ion Mobility Spectrometry) analyzer.

The Xevo G2-XS Q-TOF mass-spectrometer supports small molecules analysis, such as metabolomics or Chinese medicine studies. It combines StepWave™ ion optics with XS Collision Cell technology, to provide sensitive and high resolution mass data.

The Thermo Scientific LTQ Velos is a dual-pressure linear ion trap mass-spectrometer. It uses S-lens ion optics to increase ion transmission and trapping efficiency, ultimately offering faster scan speed and increasing overall sensitivity, which is desirable for protein identification.

Metabolomics Analyzers

Seahorse XFp Analyzers measure Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) of live cells in an 8-well miniplate format. Its quick and easy setup makes it ideal for performing routine measurements of ex-vivo metabolic phenotype and other quantity-limited samples.

The Biolog OmniLog Phenotype MicroArray (PM) Incubator Reader allows users to do PM for microbial and mammalian cells.

Protein Imaging & Western Systems

Milo™ can measure protein expression of around 1000 single cells in a single run, in which the heterogeneity of the samples can be profiled.

Odyssey is an infrared laser-based instrument for conducting Western blotting without the need for films, a darkroom, or any chemiluminescent substrates. It also offers low background fluorescence, enabling the detection of low-picogram targets with near-infrared fluorescence.

Sally Sue™ let users separate and analyze proteins by size from 2-440kDa. Users can load as little as 5μl of samples with a protein concentration of 0.2μg/μl, and run up to 96 samples in one experiment.

The ChemiDoc Touch system is a compact instrument for quantitative imaging of gels and membranes. It can detect a wide range of dyes and fluorophores.


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