
1. Protein Identification
This service incorporates both peptide mass fingerprinting (PMF) and MS/MS peptide fragmentation. The sample (single protein spot/band) is digested with trypsin and the digested fragments are recorded. A list of fragments (“fingerprint”) is generated. From the list, the most abundant peptide ions are selected for MS/MS analysis. The fragmentation produces a series of sequence ions that are used for database searching. The protein is identified by matching the experimental results with the theoretical fragmentation patterns in the MASCOT database.
a) Full service for Protein Identification (with sample preparation)
In-gel Digestion, Extraction, ZipTip® Desalting, MALDI-TOF/TOF (PMF+MS/MS) and Database Search
b) Protein Identification* (without sample preparation)
MALDI-TOF/TOF (PMF+MS/MS) and Database search
*Sample spotted on target plate
2. Accurate Mass Measurement
This service provides accurate mass measurement of protein(s) in the sample.
a) Accurate Mass Measurement
ZipTip® Desalting and MALDI-TOF Manual export of MS spectrum and peak list data (in Excel format)