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Mass Spectrometry Service

About

The facility offers mass spectrometry service with the following equipment, on a first-come-first-served basis:

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Services Provided:

1. Shotgun Protein Identification

  • In-solution tryptic digestion and desalting service available

  • Database search using Proteome Discoverer software


2. Protein Quantification

  • Label-free Quantification

  • Parallel Reaction Monitoring (PRM)

  • Labeling Quantification by MS2 (up to 18-plex for TMT labels)

  • Labeling Quantification by RTS-SPS-MS3 (up to 18-plex for TMT labels)

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3. Intact Protein Accurate Mass Measurement (<50kDa)

  • LC separation of proteins followed by accurate mass measurement

  • Mass deconvolution available

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4. Direct infusion MS analysis for high purity samples

  • HCD, CID, ETD fragmentation modes

  • Multi-stage MS (MSn) analysis available

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Services Provided:

1. Shotgun Protein Identification

  • In-solution tryptic digestion and desalting service is available

  • Database search using PEAKS Studio software


2. Protein Quantification

  • Label-free Quantification

 

3. Lipidomics

  • Database search using Bruker MetabScape software

How to apply for the service?

Please fill in the corresponding form in FULL and submit sample(s) during office hours.

Please refer to the form-filling guidelines for all three instruments here.

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Details regarding sample submission can be found here.

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For inquiries, please contact Ms. Joyce Wong at joycepswong@ust.hk or Ms. Haity Ngan at haityn@ust.hk.

FAQ

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