Mass Spectrometry Service

About

The facility offers mass spectrometry service with the following equipment, on a first-come-first-served basis:

Orbitrap Eclipse 1.png

Thermo Scientific Orbitrap Eclipse Tribrid
Mass Spectrometer 

Services Provided:

1. Shotgun Protein Identification

  • In-solution tryptic digestion and desalting service available

  • Database search using Proteome Discoverer software


2. Protein Quantification

  • Label-free Quantification

  • Parallel Reaction Monitoring (PRM)

  • Labeling Quantification by MS2 (up to 18-plex for TMT labels)

  • Labeling Quantification by RTS-SPS-MS3 (up to 18-plex for TMT labels)

​

3. Intact Protein Accurate Mass Measurement (<50kDa)

  • LC separation of proteins followed by accurate mass measurement

  • Mass deconvolution available

​

4. Direct infusion MS analysis for high purity samples

  • HCD, CID, ETD fragmentation modes

  • Multi-stage MS (MSn) analysis available

DSC05484_edited.jpg

Bruker timsTOF Pro Mass Spectrometer
(Powered by PASEF) 

Services Provided:

1. Shotgun Protein Identification

  • In-solution tryptic digestion and desalting service is available

  • Database search using PEAKS Studio software


2. Protein Quantification

  • Label-free Quantification

 

3. Lipidomics

  • Database search using Bruker MetabScape software

P10929_01_029.JPG

Bruker Daltonics UltrafleXtreme MALDI TOF/TOF Mass Spectrometer 

Services Provided:

1. Protein Identification

​This service incorporates both peptide mass fingerprinting (PMF) and MS/MS peptide fragmentation. The sample (single protein in solution) is digested with trypsin and the digested fragments are recorded. A list of fragments (“fingerprint”) is generated. From the list, the most abundant peptide ions are selected for MS/MS analysis. The fragmentation produces a series of sequence ions that are used for database searching. The protein is identified by matching the experimental results with the theoretical fragmentation patterns in the MASCOT database.​

​

a) Full service for Protein Identification (with sample preparation)

In-solution Digestion, Desalting, MALDI-TOF/TOF (PMF+MS/MS) and Database Search

 

b) Protein Identification* (without sample preparation)
MALDI-TOF/TOF (PMF+MS/MS) and Database search

*Sample spotted on a target plate

 

​

2.  Accurate Mass Measurement

This service provides accurate mass measurement of protein(s) in the sample.


a) Accurate Mass Measurement
Desalting and MALDI-TOF Manual export of MS spectrum and peak list data (in Excel format)

How to apply for the service?

Please fill in the corresponding form in FULL and submit sample(s) during office hours.

Please refer to the form-filling guidelines for all three instruments here.

​

Details regarding sample submission can be found here.

​

For inquiries, please contact Ms. Joyce Wong at joycepswong@ust.hk or Mr. Clarence Cheng at bcrfyhcheng@ust.hk.

FAQ