Mass Spectrometry Service

Bruker timsTOF Pro Mass-spectrometer
(Powered by PASEF) 

Services Provided:

1. Shotgun protein identification (In gel/ In solution tryptic digestion and desalting service is available)
2. Protein quantification (Labelling/ Label free Quantification)

Thermo Fisher Scientific LTQ Velos Dual-pressure Ion Trap Liquid Chromatography Mass-spectrometer 

Services Provided:

1. Analysis for molecular weight determination / confirmation 
2. Tandem mass spectrometry (MS/MS) for structure elucidation
3. Screen for possible post-translational modifications
4. LC/MS/MS for qualitative analysis of proteomic samples

Bruker Daltonics UltrafleXtreme MALDI TOF/TOF Mass-spectrometer 

Services Provided:

1. Protein Identification

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This service incorporates both peptide mass fingerprinting (PMF) and MS/MS peptide fragmentation. The sample (single protein spot/band) is digested with trypsin and the digested fragments are recorded. A list of fragments (“fingerprint”) is generated. From the list, the most abundant peptide ions are selected for MS/MS analysis. The fragmentation produces a series of sequence ions that are used for database searching. The protein is identified by matching the experimental results with the theoretical fragmentation patterns in the MASCOT database.​

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a) Full service for Protein Identification (with sample preparation)

In-gel Digestion, Extraction, ZipTip® Desalting, MALDI-TOF/TOF (PMF+MS/MS) and Database Search

b) Protein Identification* (without sample preparation)
MALDI-TOF/TOF (PMF+MS/MS) and Database search

*Sample spotted on target plate

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2.  Accurate Mass Measurement

This service provides accurate mass measurement of protein(s) in the sample.

a) Accurate Mass Measurement
ZipTip® Desalting and MALDI-TOF Manual export of MS spectrum and peak list data (in Excel format)