Leica Next Gen Stellaris
White Light Laser Confocal Microscope

STELLARIS is a confocal microscope that holds a white light laser for excitation. Excitation is adjustable from 485 - 790 nm, allowing users to perfectly match the excitation profile of their fluorescent dyes, which benefits multicolor experiments by minimizing spectral crosstalk and excitation of unwanted fluorophores. On the other hand, users can select excitation wavelengths not at the peak of the fluorophore’s spectrum to reduce photobleaching during prolonged live cell imaging.

 

In addition, the system utilizes HyD detectors, which provide higher photon detection efficiency (PDE) than conventional PMT detectors, extremely low dark noise, and sensitive spectral detection from 410 - 850 nm. These characteristics make way for gentle live cell imaging and give images with enhanced brightness, resolution and contrast.

 

What's more, different modules and features on the system and software benefit users in different ways: for example, Lightning resolves details down to 120 nm (xy), giving super-resolution images; Dynamic Signal Enhancement (DSE) increases signal-to-noise ratio in live cell imaging without sacrificing cell viability, acquisition speed and image quality; TauSense contains tools that work off of fluorescence lifetime for image quality improvement, fluorescent species separation etc.; Rare Event Detection allows users to set up an AI workflow to automatically capture high resolution images of infrequent interesting targets, from a low-resolution overview scan; Spectraplex helps unmix multicolor samples, ensuring reliable signal quantification.