Resources

00:00 Introduction 01:41 Clearing waste tank 03:41 Refilling ethanol tank 05:17 Checking sheath tank 05:45 Logging into computer and Bookitlab 06:52 Opening Terraterm, turning on cytometer & lasers, opening software 09:28 Fluidics startup

00:00 Selecting cytometer configuration and starting stream 03:40 Preparing for CST 04:55 Running CST 10:00 Reading CST reports and troubleshooting 11:30 Readjusting stream after CST and enabling Sweet Spot

00:00 Preparing for Accudrop 02:47 What is drop delay 03:30 Opening Accudrop template and running Accudrop 09:05 Optimizing side stream position

00:00 Creating experiment and sample loading 02:25 Adjusting detector parameters and display count and recording 04:58 Defining populations, showing population hierarchy 06:25 Changing sample, renaming populations 08:25 Setting up sort layout, starting sort and monitoring sort 11:57 Changing ND filter 12:32 Saving PDF, exporting experiments and fcs files, uploading files to server

00:00 Cleaning up after use 02:15 Fluidics shutdown, computer shutdown, cytometer shutdown 06:45 Dealing with nozzle clog